O-LCA is envisioned for organizations of all sizes, both public and private, in all sectors, and all over the world. We devoted special efforts to make the new methodology operative for all of them. Furthermore, the guidance document defines the so-called “experience-based implementation pathways” and provides particular methodological support according to previous experience of the organization with other environmental tools. Eleven experiences of the so-called “First Movers” of O-LCA illustrate the process and benefits that the methodology could bring to organizations. Eight sectors and four regions are represented in the case studies.